Anti-Hyperglycemic Activity of Aqueous Extract of Lawsonia Inermis Leaf in Alloxan-Induced Diabetic Wistar Rats (Anti-Hyperglycemic Activity of Aelil)

Abstract

Background: Alterations in metabolism of fuel molecules in diabetes increase blood glucose. This causes long-term complications in many organs. Currently, available treatment options to maintain glycemic control are accompanied by various side effects. Therefore, there is need to develop newer antihyperglycemic agents of plant origin with no side effects.
Objective: This study aimed to evaluate the anti-hyperglycemic effects of aqueous extract of Lawsonia inermis leaf in diabetic rats.
Methods: Thirty albino rats of an average weight 203.21 ± 6.57 g were randomly divided into six groups. Diabetes was induced through intraperitoneal administration of 150 mg/kg body weight of alloxan in all animal groups except one. Group A, non-diabetic rats, and group B, a diabetic control group were treated with 0.5 mL of normal saline while group C was treated with 50 mg/kg body weight metformin. Groups D, E and F were administered daily dose of 200, 400, and 600 mg/kg body weight of aqueous extract of Lawsonia inermis leaf (AELIL) respectively for a period of 3 weeks. Parameters monitored are fasting blood glucose using glucometer, body weight, serum chemistry and liver enzyme markers.
Results: Administration of AELIL showed a significant (p < 0.05) reduction in fasting blood glucose of diabetic rats in a dose dependent manner. Also, administration of AELIL has no negative effect on the body weight, serum chemistry and liver marker enzymes of diabetic rats. The AELIL showed no observable toxicity.
Conclusion: AELIL possesses antidiabetic activity and safe for consumption.