Phytochemical Screening and Antibacterial Activity of Mangifera indica Extracts

Abstract

Mangifera indica leaves are used for the treatments of various ailments in folklore medicine. This research was aimed to determine phytochemical composition and antibacterial activity of leaves extracts of Mangifera indica. Powdered leaves of Mangifera indica were extracted with water, ethanol and chloroform solvents via percolation method. The extracts were tested for antibacterial activity against clinical isolates of Salmonella Typhi, Salmonella Paratyphi A and Salmonella Paratyphi B using agarwell diffusion method. The extracts were further subjected to phytochemical tests for the presence of secondary metabolites using standard procedures. Results of the sensitivity test showed that highest zone of inhibition was observed in ethanolic extract with 13mm for S. Paratyphi A, 11mm for S. Paratyphi B, and 10mm for S. Typhi, followed by aqueous extract with 11mm for S. Paratyphi B and 10mm for S. Typhi. S. Paratyphi A, was resistant to both aqueous and chloroform extract of M. indica while S. Paratyphi B was only resistant to chloroform extract. Results of phytochemical screening indicated the presence of alkaloids, flavonoids, steroids, tannins and phenols.