Preclinical Hba1c level studies of Makardhwaj Ras and Maha Sankha Bati after chronic administration to male Sprague-Dawley rats

Abstract

Background: Makardhwaj Ras (MRS) and Maha Sankha Bati (MSB) are Ayurvedic preparations used as traditional medicines for different clinical indications in the rural population. MRS and MSB are used in the debility and sprue, respectively. In this study, the effect of these preparations was evaluated on HbA1c levels (percentage) in rats after chronic feeding. The acute toxicological test of MRS and MSB separately did not record death or signs of toxicity even at the highest dose of 4000 mg/Kg body weight. In the chronic toxicological evaluation, Ayurvedic medicinal preparations MRS and MSB were administered chronically to the male Sprague-Dawley rats at a dose of 40 mg/kg and 400 mg/kg for MRS and MSB, respectively, to determine the level of HbA1c (percentage). After 28 days of chronic administration of MRS and MSB, the level of HbA1c level was determined in the blood and found that both preparation MRS and MSB increased the level of HbA1c (percentage) in the blood of the rat. MRS and MSB increased the level of HbA1c in rats, which is a reliable indicator of diabetes mellitus. Therefore, precaution is recommended in the administration of MRS and MSB for a prolonged period.