A Comparative Study on Polyphenol, Flavonoid Content, Antioxidant and AntiInflammatory Capacity of Different Solvent Extract from Portulaca oleracea in Carrageenan-Induced Paw Edema in Mice

Abstract

Portulaca oleracea L., belonging to the Portulacaceae family, is a succulent annual plant, that has been used as  vegetables and folk medicine in many Asian countries. There is no systematic and comprehensive research on the  comparison of phytochemical quantification and biological activities from various extracts of this plant. This study  examined the total polyphenol and flavonoid content of various Portulaca oleracea extracts and assessed their  antioxidant and antiinflammatory properties. Comparing four extracts (methanol, aqueous, ethyl acetate, n-hexane) from P. oleracea, methanolic extract (ME) showed the greatest total polyphenol (60.52±1.88, mg GAE/g DW) and  flavonoid content (44.78±1.17 mg QE/g DW). Thus, across all activities examined, ME had the strongest in vitro  antioxidant (IC50=43.52 g/mL for DPPH, IC50=9.71 g/mL for FRAP), and anti-inflammatory effects (80% protein  denaturation and 74.81% protection of HRBC membrane). Remarkably, after treatment of ME for 5 h, ME has significantly reduced paw volume (0.098 mL) and thickness (0.62 mm) on carrageenan-induced inflammation (hind paw  edema model), compared to the inflamed model (0.19 mL and 1.52 mm). Moreover, ME helps protect the inflamed site in  mice based on its ability to reduce malondialdehyde (MDA) and increase glutathione (GSH). The applications of ME in  the treatment of inflammatory illnesses and oxidative damage are pharmacologically supported by our results. Also, 60  minutes after ingestion of ME, it dramatically raised the plasma levels of polyphenols and flavonoids as well as the  antiradical capacity in healthy mice. Our results showed that ME consumption can support in vivo diet-related health  advantages.