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Estimation of protein degradationin rumen by three methods
Abstract
The rumen degradability of protein in diets containing maize straw, fish meal and 0, 30 and 60% maize grain was estimated in three ways: (i) from the difference between the total non-ammonia Nand microbial N entering the duodenum over a 24-hour period using 35S and DAPAas microbial markers, (H) from the disappearance of N from nylon bags in the rumen and the rate of outflow of rumen solids, and (iii) from the solubility of the feed N in a bicarbonatephosphate buffer. The values obtained by methods (i) and (Iii) decreased as the proportion of grain in the diet increased. Those given by method (H)showed no definite trend.
Voerprote'ienafbraak in die herkouer rumen van diete bestaande uit mieliestrooi, vismeel en 0, 30 en 60% mieliegruis is met behulp van die volgende metodes bepaal: (i) die verskil tussen die totale NAN en mikrobe N in duodenale inhoud deur gebruik te maak van 35S en DAPA as mikrobe merkers, (ii) die verdwyning van N uit nylon sakkies in die rumen, gekorrigeer met die vloei van vastestowwe uit die rumen uit, (Hi) en die oplosbaarheid van voer N in 'n bikarbonaat-fosfaat buffer. Die resultate verkry met metodes (i) en (iii) toon 'n afname in proteren afbraak met 'n toename van mieliegruis in die dieet, terwyl metode (ii) geen definitiewe tendens toon nie.
Keywords: Rumen, Protein degradation, estimation