Distribution of urease producing bacteria in the t I . r raDDlt caecum

Abstract

The distributiono f urease-producinbga cteriaw as examinedi n lumenal and mucosal-associatedp opulationso f the rabbit caecum.T he total numbero f bacteriar ecoveredo n a nonspecific, s emi-definedm edium were 1,7 x 10ep er g from lumen contents and 2,0 x 10sp er g of washed,h omogenizedc aecal wall. Urease activity was shown by 5,3% of strains isolated from lumen contents and 21,770m ucosal-associateds trains. A higher proportion of bacteria associated with the caecal wall (63%) were facultative compared with 20% from the caecalcontent.

Die verspreidingv an bakterie€w at ureasep roduseeri s onder' soek in die buisholtee n slymvliesg eassosieerdep opulasies van die konyn se sakderm. Die totale aantal bakteried wat her' win is op 'n nie-spesifiekes,e mi-gedefinieerdme edium was 1,7 x 10e per g van die buisholte inhoud en 2,0 x 105 per g van gewas en gehomogeniseerdesa kdermwand.U reasea ktiwiteit is aangetoon vir 5,370 van die stamme geisoleer uit die buisholte inhoud en 21,7oh van die slymvlies geassosieerde stamme. 'n Groter gedeelte van die bakterie€ wat geassosieer word met die sakdermwand (63%) was fakulatief in vergelyking met die 20o/ov a'fid ie sakdermi nhoud.

Keywords: Urease, rabbit caecum, anaerobic bacteria, mucosalassociated flora