Media lacking nrmen fluid for enumeration of rumen bacteria

Abstract

A medium without rumen fluid but containing trypticase, hemin and volatile fatty acids was developed for the enumeration of rumen bacteria. Counts of total culturable and amylolytic bacteria were as high or higher than those obtained using medium CC (Zeigler-Leedle & Hespell, 1980)or medium 10 (Caldwell & Bryant, 1966).However, counts of cellulolytic bacteria were low probably owing to the absence of blending to remove bacteria from ingesta particles. 

'n Medium sonder rumenvloeistof, maar wat triptikase, hemien en vlugtige vetsure bevat, is ontwikkel vir die vermeerdering van die bakteriee van die grootpens. Tellings van totale kweekbare en amilolitiese bakteriee was net so hoog of hoer as die verkry met medium CC (Zeigler-Leedle & Hespell, 1980) of medium 10 (Caldwell & Bryant, 1966).Die tellings van sellulolitiese bakteriee was egter laag, waarskynJik as gevolg van die afwesigheid van menging om die bakteriee van die verteringsmateriaal te verwyder.

Keywords: Rumen ecology, rumen bacteria, enumeration of rumen bacteria, selective culture media, culture techniques, rumen population, cellulolytic bacteria, amylolytic bacteria