Genetic Diversity Of Andropogon Species With Different Microsatellite Markers For Better Selection And Improvement

Abstract

Pastures have over the years been selected for important traits and their breeding has resulted in some high yielding forage grasses with great adaptive and population expansion traits. The research was carried out to study genetic diversity of nine Andropogon spp. sourced from Remo zone of Ogun State using four microsatellite markers (Phil227562, Xcup63, CTM59, and Xcup14). Genomic DNA was extracted from succulent leaf part of Andropogon grass using DNA extraction procedure of Zymo spin™ technology. Every one of the loci–populace is in with Hardy–Weinberg equilibrum. A sum of eight alleles were noticed and the quantity of alleles per locus has an estimation of 2.00, while the number of alleles ranged from 1.528 (Phil227562) to 1.946 (Xcup63). The viable number of alleles was lesser than noticed number at all the loci. The Shannon's Information Index differed among the loci with 0.530 (Phil227562) to 0.679 (Xcup63) though Inbreeding coefficient across the Andropogon grass clones was negative (mean = - 0.429), going from 0.07 (Phil 227562) to 0.714 (Xcup63) with 100% polymorphic loci. The noticed heterozygosity was higher than expected heterozygosity for all markers of the grass considered.