Article Sidebar
Published:
Aug 31, 2021Keywords:
Article Details
NAJ supports free online communication and exchange of knowledge as the most effective way of ensuring that the fruits of research and development practice are made widely available. It is therefore committed to open access, which, for authors, enables the widest possible dissemination of their findings and, for readers, increases their ability to discover pertinent information. The Journal adopts and uses the CC: BY license and is open access. This license lets others distribute, remix, adapt, and build upon your work, even commercially, as long as they credit you for the original creation. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the Journal’s published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work. Copyright for articles published in this Journal is retained by the Journal.
Main Article Content
Cell Suspension Culture of Mucuna pruriens For Production and Improvement of L-3, 4-Dihydroxy Phenylalanine Concentration
Abstract
The production and improvement of L-3, 4-dihydroxy phenylalanine (L-Dopa) content in Mucuna pruriens was carried out through cell suspension culture. Thirty days old healthy, friable and soft calli derived from leaf and stem of two varieties of M. pruriens: (IIHR Selection 3 and Arka Dhanvantari) were used for the cell suspension in Murashige and Skoog’s liquid medium supplemented with IAA (1.0mg/L), NAA (1.5mg/L) and BAP (1.5mg/L) with treatment of elicitors: chitin and pectin (100, 150, and 200mg/L), precursor: L-tyrosine (5, 10, and 15mg/L) and ascorbic acid (250mg/L). The highest percentage of packed cell volume (PCV) (42.4 ± 0.1) was observed in leaf-derived callus of AD variety with treatment L4: MS + NAA (1.5mg/L) + Pectin (100mg/L) + ascorbic acid (250mg/L). From the HPLC analysis, treatment T3c: MS + IAA (1.0mg/L) + L-tyrosine (15mg/L) + ascorbic acid (250mg/L) recorded highest peak area percentage of 31.19 at retention time (RT) of 2.36 against control (1.39) at 2.35 RT. L-Dopa concentration was observed to increase with increase in elicitor and precursor treatment. This shows that L-Dopa can be produced from natural source in desired concentration through cell suspension culture.
