Main Article Content

Effect of added Theobroma cacao Leaves Extract on the Oxidative Stability of Refined Palm Olein during Accelerated Storage


Gires Boungo Teboukeu
Serge Cyrille Houketchang Ndomou
Hilaire Macaire Womeni

Abstract

This study was conducted to evaluate the antioxidant potential of Theobroma cacao leaves extract on the oxidative stability of palm olein (PO). The plant  material was extracted with methanol and its total phenolic, flavonoid contents and radical scavenging activity were evaluated, followed by the  identification of some phenolic antioxidants by high-performance liquid chromatography (HPLC). The cocoa leaves extract was added in palm olein at  concentrations of 500–2000 ppm. Oil containing butylhydroxytoluene (BHT) and oil without antioxidants served as positive and negative controls  respectively. The oxidative stability of these oil samples was evaluated by determining their induction times on Rancimat (at 110°C) and measuring their  oxidative state using the Schaal oven for a period of 46 days at 70°C (8 hours of heating per day) for measurement of their primary and secondary  oxidation products. Results showed Theobroma cacao leaves extract to be rich in phenolic antioxidants and to be a good radical scavenger. Caffeic acid  and vanillic acid were the phenolic antioxidants detected by HPLC. The induction times of palm olein supplemented with the extract were found to be  ranged between 26.0 –27.99 hours, values significantly (p < 0.01) higher than that of the negative control. The extract, at all concentrations, were also  found to be efficient in delaying oxidation of palm olein during 46 days storage at 70°C. It can be concluded that Theobroma cacao leaves extract can be  a natural source of antioxidants for the stabilization of palm olein. 


Journal Identifiers


eISSN: 2663-1741
print ISSN: 2664-5513