Article Sidebar
Published:
May 7, 2016Keywords:
Article Details
Open access articles published in the Journal of Agriculture, Science and Technology are under the terms of the Creative Commons Attribution (CC BY) License which permits use, distribution and reproduction in any medium, provided the original work is properly cited. The CC BY license permits commercial and non-commercial re-use of an open-access article, as long as the author is properly attributed.
Copyright on any research article published in the Journal of Agriculture, Science and Technology is retained by the author(s). The authors grant the Journal of Agriculture, Science and Technology with a license to publish the article and identify itself as the original publisher. Authors also grant any third party the right to use the article freely as long as its original authors, citation details and publisher are identified.
Use of the article in whole or in part in any medium requires proper citation as follows:
Title of Article, Names of the Author, Year of Publication, Journal Title, Volume (Issue) and page. Links to the final article on the JSRE website are encouraged.
The Creative Commons Attribution License does not affect any other rights held by authors or third parties in the article, including without limitation the rights of privacy and publicity. Use of the article must not assert or imply, whether implicitly or explicitly, any connection with, endorsement or sponsorship of such use by the author, publisher or any other party associated with the article.
For any reuse or distribution, users must include the copyright notice and make clear to others that the article is made available under a Creative Commons Attribution license, linking to the relevant Creative Commons web page. Users may impose no restrictions on the use of the article other than those imposed by the Creative Commons Attribution license.
To the fullest extent permitted by applicable law, the article is made available as is and without representation or warranties of any kind whether express, implied, statutory or otherwise and including, without limitation, warranties of title, merchantability, fitness for a particular purpose, non-infringement, absence of defects, accuracy, or the presence or absence of errors.
Main Article Content
An Aspergillus Isolate and Its Secondary Metabolites From Lake Elmentaita in Kenya
Abstract
The soda lakes provide a high biodiversity of halophilic and alkaliphilic fungi. The fungus in this study was isolated from sediments collected from Lake Elmentaita in Kenya. The effect of pH and sodium chloride on growth was done on Malt Extract Agar (MEA) and Potato Dextrose Agar (PDA) media. Fermentation of fungal isolate in 500 ml conical flasks was done in fourteen days’ duration on a shaker. Extraction of crude fermentation solution was done using the solvents, Ethyl acetate and hexane in the ratio 2:1 respectively. Molecular characterization of the fungus was done using the18s rDNA Gas-chromatography-Mass spectrophotometry (GC-MS) analysis revealed seventeen secondary metabolites produced by the fungus. The fungus grew well at alkaline pH of 9 to12 and a temperature range of 26°C to 30°C. Growth was only observed on 0% to 10% sodium chloride. The culture-dependent assays and GC-MS analysis showed that the fungus produces bioactive compounds that can be applied in biotechnology research. Moreover, molecular analysis grouped the isolate as a close relative to the genus Aspergillus.
