Sirtuin 1 gene rs2273773 C >T single nucleotide polymorphism and protein oxidation markers in asthmatic patients

Abstract

Background: Sirtuin-1 (SIRT-1), a protein has been found to protect the cells against oxidative stress due to its deacetylase activity. In this investigation, we aimed to study SIRT-1 gene rs2273773 C >T single nucleotide polymorphism and markers of serum protein oxidation (protein carbonyl and sulfhydryl groups) in asthmatic patients.

Subjects and methods: 120 asthmatic patients and 120 healthy controls were genotyped for SIRT- 1 gene rs2273773 C > T SNP using polymerase chain reaction – confronting two pair primer method (PCR-CTPP). Serum protein carbonyl and sulfhydryl groups were measured using colorimetric methods.

Results: SIRT-1 gene rs2273773 C> T SNP genotyping revealed that the TT genotype was significantly higher in the patients compared to the controls (P < 0.05), while there were no significant differences regarding the genotypes TC and CC between the patients and the controls (P> 0.05). T allele was significantly higher in the patients compared to the controls (P =0.017). The distribution of the genotypes didn’t differ among the atopic and the non-atopic asthmatic patients, also no difference was found in the genotype distribution according to the severity of asthma (P >0.05). Serum protein carbonyl group concentration was significantly higher in the patients compared to the controls (P < 0. 001), while serum protein sulfhydryl group content decreased significantly in the patients compared to the controls (P < 0.0001). No differences in markers of protein oxidation according to SIRT-1 gene rs2273773 C > T genotype were found.

Conclusion: SIRT-1 gene rs2273773 C> T SNP was associated with asthma but not with protein oxidation markers in Egyptian population.

Keywords: Asthma; Gene polymorphism; Protein oxidation; Sirtuin 1; Sulfhydryl group