Association of glutathione-S-transferase P1 (GSTP1)-313 A> G gene polymorphism and susceptibility to endometrial hyperplasia among Egyptian women

Abstract

Background: Endometrial hyperplasia (EH) occurs when the endometrium, the lining of the uterus, becomes too thick, causing abnormal uterine bleeding. In some cases, it can lead to endometrial carcinoma if untreated. Glutathione-S-transferases (GSTs) enzymes have a role in the metabolism of a lot of disease-causing carcinogens and mutagens that are present in environments of human. GSTP1–313 A >G gene polymorphism was associated with significantly high risk of endometrial carcinoma in some reports. The aim of this work is to assess the association of this polymorphism with the susceptibility of EH among a sample of Egyptian women.
Subjects and methods: This study included 84 unrelated EH Egyptian women who were compared to 134 healthy controls from the same locality. For all subjects, DNA was genotyped for GSTP1–313 A> G (Ile105Val) polymorphism using the PCR–RFLP technique.
Results: The frequency of GSTP1–313 AG and GG genotypes was noted to be significantly higher among most of the cases with EH compared to controls (79.8% vs. 42.0%, OR= 8.63, 95% CI =4.53–16.46, p< 0.001). Also the frequency of the GSTP1–313 G allele was significantly higher among most of the cases compared to controls (24.9% vs. 16.8%, OR =3.72, 95% CI= 2.39–5.79, p< 0.001). Furthermore, there was a significant increase of endometrial thickness in the EH women compared to controls (p< 0.001).
Conclusions: GSTP1–313 G allele carriage was associated with susceptibility of EH among a sample of Egyptian women.

KEYWORDS: Endometrial hyperplasia; Gene polymorphism; GSTP1; Ile105Val