Association of CYP3A4 and CYP3A5 polymorphisms with Iranian breast cancer patients

Abstract

Background: Polymorphisms of different gene have been reported to be associated with
cancer including breast cancer. Hospitalization rate for breast cancer has increased over the years in Iran.
Aim: The aim of this study was to examine whether polymorphisms in the CYP3A4 and
CYP3A5 genes affect the risk of developing breast cancer.
Subjects and methods: The genotype distribution and allele frequencies of four CYP3A4*1A, CYP3A4*1B, CYP3A5*1 and CYP3A5*3 single-nucleotide polymorphisms were determined in 250 subjects from the general population in Ahvaz city (southwest of Iran) including 200 healthy subjects and 50 individuals affected with breast cancer.
Results and conclusion: The genotype frequency of CYP3A4*1A/*1A (A/A) in both case and control groups was 100%; however, there was no subject with either CYP3A4 * 1A/ * 1B (A/G) or CYP3A4*1B/*1B (G/G) genotype. For CYP3A5 gene, CYP3A5*3/*3mutant homozygote genotype frequency was found to be 99% (n= 198) and 98% (n= 49) in control and patient groups respectively. CYP3A5*1/*1 wild-type genotype was calculated to be 1% (n= 2) in the control group and 2% (n=1) in the case group. No. CYP3A5*1/*3 heterozygote genotype was detected in the both groups. The results showed that there was no association between breast cancer, CYP3A5 (P-value = 0.561) and CYP3A4 allele distribution.

KEYWORDS: Breast cancer; CYP3A4; CYP3A5; Polymorphism; Iranian population