The promoter region (G-800A and C-509T) polymorphisms of transforming growth factor-β1 gene among young women with recurrent urinary tract infection

Abstract

Background: Recurrent urinary tract infection (UTI) is common among young women and one of its risk factors is genetic. Polymorphisms in promoter region (G-800A (rs1800468) and C-509T (rs1800469)) of transforming growth factor-β1 (TGF-β1) gene play pivotal roles in several infection diseases but the association of these polymorphisms with recurrent UTI remains unclear. The aim of this study was to assess the correlation of TGF-β1 G-800A and C-509T polymorphisms with recurrent UTI in young women.
Subjects and methods: TGF-β1 G-800A and C-509T polymorphisms among 34 recurrent UTI patients and 34 healthy subjects, aged 15–50 years old, were evaluated with polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP) and confirmed by DNA sequencing.
Results: At position 800, genotypes showed no significant differences between recurrent UTI patients (GG 97.1%; GA 2.9%; AA 0%) and normal control (GG 97%; GA 0%; AA 2.9%) young women. Dominant and recessive model analyses did not find significant correlation between recurrent UTI patients and normal control young women. At position 509, allelic and genotypic frequencies showed no significant differences between recurrent UTI patients (CC 20.6%; CT 61.8%; TT 17.7%) and control individuals (CC 2.9%; CT 73.6%; TT 23.5%).
Conclusion: This study found that there is no strong correlation between polymorphisms of TGF-β1 G-800A and C-509T and recurrent UTI.

Keywords: SNP; Polymorphism; Urinary tract infection; Recurrent UTI; Transforming growth factor; TGF-β1