Antimicrobial activity of aloe Secundiflora against clinical isolates of Helicobacter pylori

Abstract

Objective: To evaluate the antimicrobial activity of methanol and aqueous crude extracts of Aloe secundiflora (A. secundiflora) against clinical isolates of Helicobacter pylori which is the most prevalent cause of gastrointestinal infections.
Methods: The agar diffusion method was used to determine the susceptibility of 23 clinical isolates of H. pylori to the methanol and aqueous crude extracts of A. secundiflora. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) was determined by micro well dilution method. The presence of secondary metabolites was determined. The potential bioactive compounds were identified by Gas Chromatography-Mass Spectrometry (GC-MS).
Results: Both methanol and aqueous crude extracts of A. secundiflora demonstrated antimicrobial activity with highest mean zone diameter of 28 ± 0.47 mm for methanol extracts and 11+0.81mm for aqueous extracts. Lowest recorded MIC and MBC was between 0.19-0.39mg/ml for methanol and aqueous extracts respectively. There was no statistically significance difference (p>0.05) in potency of the extracts in different isolates of H. pylori tested both in MIC and MBC. Phytochemical screening of methanol and aqueous crude extracts of A. secundiflora showed presence of secondary metabolites such as alkaloids, saponin, tannins, flavonoids, and steroids. A total of 8 bioactive compounds were identified GC-MS analysis.
Conclusion: The present study provides evidence that methanol and aqueous extracts of A. secundiflora possess inhibitory prospects against clinical isolates of H. pylori.