Optimization of protocols for DNA extraction and RAPD analysis in West African fonio (Digitaria exilis and Digitaria iburua ) germplasm characterization

Abstract

Fonio is an important indigenous grain crop of West Africa, but the extent of genetic diversity in fonio, its origin and phylogeny are not well understood. DNA markers allow precise characterization of plant germplasm accessions, but there is no literature report of their use in fonio. This paper reports the
result of protocol optimization research for DNA isolation and RAPD analyses in fonio. High quality DNA was successfully isolated and RAPD was effectively used to study genetic similarity among fonio accessions. This result might stimulate the application of DNA markers to investigate the origin and phylogeny of fonio in Africa.