Stability of wheat germ oil obtained by supercritical carbon dioxide associated with lipase ethanolysis

Abstract

Wheat germ oil was extracted using an environmental friendly solvent, supercritical carbon dioxide (SCCO2) at a semi-batch flow extraction process. The supercritical carbon dioxide (SC-CO2) extraction was carried out to extract oil at temperature of 40°C and pressure of 25 MPa. Ethanolysis was performed with 1,3-regiospecific lipase at different temperatures (40 to 70°C) to produce diglycerides and monoglycerides containing fatty acid ethyl esters. For determination of stability, wheat germ oil obtained by ethanolysis reactants was characterized by measuring the acid value, peroxide value, free fatty acid contents, thiocyanate method, DPPH radical scavenging effect and rancimat test. The optimized condition of 40°C shows the highest oil stability among the various conditions.

Keywords: Supercritical carbon dioxide, wheat germ oil, ethanolysis, immobilized lipases, oil stability

African Journal of Biotechnology Vol. 12(22), pp. 3570-3575