Genetic analysis of baculovirus resistance in lepidopteran model insect Bombyx mori L.

Abstract

In order to clarify the resistant mechanism of BmNPV in silkworm, and from negative to prove agricultural pest inheritance of virus resistance, in this study, we used the highly resistant strain NB and susceptible strain 306 as the material through the method of classical genetics experiment, and proved that the baculovirus resistance in silkworm is controlled by a pair of autosomal dominant major gene. At the same time, we used random amplification of polymorphic DNA (RAPD) random primers to screen a molecular marker which are in high linkage with the resistant trait. Validity of the molecular marker was proved in BC₁, F₂ populations, which further demonstrated that the baculovirus resistance in silkworm is controlled by a pair of autosomal dominant major gene. This can provide an effective research basis for the emergence of baculovirus resistance in pest and its resistant mechanism.

Key words: Bombyx mori, molecular markers, genetic analysis, biological control, baculovirus resistance.