Zimbabwe Veterinary Journal

Real-time PCR for detection of Theileria equi and Babesia caballi parasites in ticks

Tatenda Chiuya, Colin Musara — Wed, 07 Mar 2018 00:00:00 +0000

The tick, Dermacentor reticulatus is a vector for Babesia caballi and Theileria equi which cause equine babesiosis and theileriosis respectively. This study aimed to develop a real-time PCR screening test for Babesia caballi and Theileria equi in ticks. Adult D. reticulatus were collected from De Panne, Belgium. Nested PCR was used with B. caballi and T. equi specific adjacent hybridisation FRET probes to develop a qualitative real-time PCR. The test was used to screen DNA extracts from collected ticks. Probe sequences were based on differences in the 18S rRNA gene of the pathogens. DNA extracts from known Theileria and Babesia spp. were used as positive controls. Using melting curve analysis probes were able to detect and distinguish the B. caballi and T. equi positive controls. None of the tick samples tested positive for T. equi and B. caballi but one sample was positive for T. parva. This test is suitable for application in epidemiological surveillance of equine babesiosis and theileriosis.

Keywords: Dermacentor reticulatus; Babesia caballi; Theileria equi; real-time PCR; FRET probe

Prevalence of intestinal helminth parasites in stray dogs in urban Harare and selected rural areas in Zimbabwe

Ashley-Kate Davidson, Kalnisha Bhikha, George D. Vassilev, Solomon Dhliwayo — Wed, 07 Mar 2018 00:00:00 +0000

A total of 246 faecal samples were collected between October 2015 to February 2016, 154 from stray dogs in Harare and 92 from rural dogs in Arcturas, Goromonzi and Christon Bank. The samples were examined by flotation and sedimentation methods and helminth eggs identified and EPG counted. Of the 246 samples, 157 (63.8%) were positive for helminths. Urban stray dogs (58.4%) had a significantly (p = 0.03) lower prevalence compared to rural dogs (72.8%). Nine species were identified and Ancylostoma species (55.7%), Dipylidium caninum (15.0%) and Toxocara canis (8.1%) had the highest prevalences. The other species were Toxascaris leonina (1.6%), Alaria species (1.6%), Spirocerca lupi (0.8%), Trichuris vulpis (0.8%), Capillaria species (0.4%) and Ascaris suum (0.4%) and were all found only from rural dogs. This is the first report documenting presence of Alaria species in Zimbabwe. Public education on control of zoonotic canine helminthosis is recommended.

Keywords: Intestinal helminths, urban stray dogs, rural dogs, Harare

Contribution of Lactobacillus plantarum in fermented dairy products to food safety and public health

Colin Musara — Wed, 07 Mar 2018 00:00:00 +0000

Strains of Lactobacillus plantarum recently isolated from artisanal fermented milks and milk products include L. plantarum AMA-K, L. plantarum KLDS1.0391, L. plantarum ST27, L. plantarum LL441, L. plantarum ST8K and L. plantarum BR12. The isolates exhibited in vitro antimicrobial activity against saprophytic and enteropathogenic bacteria which include Listeria monocytogenes, Staphylococcus aureus, Bacillus cereus, Escherichia coli, Clostridium difficile, Clostridium perfringens, Klebsiella pneumoniae, Enterococcus faecalis, Pseudomonas fluorescens, Salmonella typhimurium, Salmonella typhi, Salmonella enterica and Shigella flexneri. The broad antimicrobial spectrum affords ample opportunity for use of L. plantarum strains as food preservatives and probiotics in dairy products. In vivo, health benefits of L. plantarum reported in humans include increased fermentation in the hindgut, reduced intestinal load of diarrhoea-causing Enterobacteriacea, predominance of ingested L. plantarum over enteric pathogens in the intestinal microflora and reduced incidence of Clostridium difficile-associated diarrhoea.

Compound oblique distal diaphyseal tibiotarsal fracture in a 5 year old peacock

R.A. Ajadi, F.E. Ojeblenu, M.T. Onadeji, F.E. Sobayo, O.S. Aduloju, O.M. Nasir — Wed, 07 Mar 2018 00:00:00 +0000

A five year old peacock presented with complete lameness of the right hind limb was diagnosed of having a compound oblique comminuted fracture of distal one-third of right tibiotarsal bone based on radiographic findings. The fracture was first managed with plaster cast device. However, the bird was presented three weeks later due to lamness on the same leg. Open reduction and internal fixation with a bone plate was done. The bird was premedicated with intramuscular injection of butorphanol (0.5mg/kg) and Xylazine (2mg/kg), while general anaesthesia was induced and maintained with midazolam (0.3mg/kg) and Ketamine (5mg/kg) intramuscularly. Following open reduction, the fracture was reduced and immobilized with improvised paediatric reconstruction plate. Post reduction radiograph revealed adequate reduction and proper implant position. The fracture healed without any complication. Although materials used for canine practice could be adapted for use in birds, the smaller size of some avian patients is a major limitation.

Imperforate anus in a male Friesian-Holstein calf: Case report and review of literature

Wed, 07 Mar 2018 00:00:00 +0000

A case of imperforate anus in a 3-day old male Friesian-Holstein calf was presented and treated in Bugesera district, Rwanda. The condition has rarely been reported in Africa. A brief review of the etiology, epidemiology, diagnosis, surgical and post-surgical management and classification of anorectal malformations (ARM) as they occur in man and domestic animals with emphasis on imperforate anus is hereby presented. We focus on the usage of the terms imperforate anus, atresia ani and make a distinction between these terms and other ARMs. With emphasis placed on the nomenclature of ARMs and the distinction between usage of the terms imperforate anus and atresia ani, we propose that the two terms be viewed as different and standard scientific nomenclature of Type I –IV ARM be used consistently to avoid confusion. If the terms atresia ani and imperforate anus should be used, they must be used correctly and consistently.