Implication of Copper II Ion in the Modelling of Growth Kinetics of TBT-Resistant Klebsiella sp. FIRD 2

Abstract

Tributyltin (TBT) is one of the most toxic substances ever deliberately introduced into the marine environment. It is an organotin compound mostly used as a wood preservative, pesticide, bactericide, PVC stabilizer, fungicide, and antifouling biocide in boat and ship paints to prevent attachments of marine organisms on the hull surface. We studied the TBT-resistant bacterium by Klebsiella sp. FIRD 2 containing copper II ion and modeled it using several kinetic models such as Monod, Haldane, Luong, Aiba, Teissier, Yano, and Webb, and estimated the accuracy of the fitted model using statistical analysis such as coefficient of determination (R²), adjusted coefficient of determination (adj R²), and root mean square error (RMSE). The Aiba model was the best fit to the experimental growth kinetics data determined and provided a very good fit. The estimated values for the Aiba constants in this work, such as maximal growth rate, half inhibition constant, and half saturation constant rate, designated as umax, ki, and ks, were 0.1265 hr⁻¹, 8.061 mg/L, and 0.8300 mg/L, respectively. The true umax, where the gradient for the slope is zero for the Aiba model, was approximately 0.061093 hr⁻¹ at 1 mg/L copper.