Assessment of Serum Biochemical Indices of Albino Rats Induced with Naja nigricollis Venom and Treated with Parkia biglobosa Stem Bark

Abstract

Assessments of Serum Biochemical Indices of Albino Rats induced with Naja nigricollis venom treated with Parkia biglobosa stem bark were conducted and evaluated. A total of 42 healthy adult Albino rats of both sexes and average weight 153-275g were used for the experiment. The venom was collected in low light condition at ambient temperature by using a short-acting general anesthesia (Halothane) and kept at 4°C. The rats were injected with venom at 0.04 mg followed by conventional antivenom (1 ml), and the plant extract was administered at different doses of 300, 500, and 700 mg/kg, respectively. The animals were sacrificed, and 5 ml of blood samples were collected from each rat within 24 hours and tested for ALP, ALT, AST, total protein, and Albumin. The LD50 was determined using Lorke’s method and found to be greater than 500 mg/kg per oral. The results showed increases and decreases in ALT, ALP, and AST, while total protein and Albumin were normal. The stem bark extract of Parkia biglobosa can be said to possess anti-snake venom activity, which neutralizes the toxic effects of N. nigricollis venom. The potent snake venom neutralizing capacity of this plant extract is recommended for therapeutic purposes in case of snake bite.