M. Mathias
Department of Microbiology, Parasitology and Biotechnology, College of Veterinary Medicine and Biomedical Sciences, Sokoine University of Agriculture, P. O Box 3019, Morogoro, Tanzania, Tanzania Veterinary Laboratory Agency, Centre for Infectious Diseases and Biotechnology, P. O Box 9254, Dar es salaam, Tanzania and SACIDS Foundation for One Health, Africa Centre of Excellence P. O. Box 3297, Morogoro, Tanzania
P. Wambura
Department of Microbiology, Parasitology and Biotechnology, College of Veterinary Medicine and Biomedical Sciences, Sokoine University of Agriculture, P. O Box 3019, Morogoro, Tanzania
S. Kimera
SACIDS Foundation for One Health, Africa Centre of Excellence P. O. Box 3297, Morogoro, Tanzania and Department of Veterinary Medicine and public Health, College of Veterinary Medicine and Biomedical Sciences, Sokoine University of Agriculture, P. O Box 3021, Morogoro, Tanzania
R. Fyumagwa
Tanzania Wildlife Research Institute, P. O. Box 661, Arusha, Tanzania
S. Kandusi
Department of Microbiology, Parasitology and Biotechnology, College of Veterinary Medicine and Biomedical Sciences, Sokoine University of Agriculture, P. O Box 3019, Morogoro, Tanzania
R. Sallu
Food and Agriculture Organization, P. O. Box 2, Dar es Salaam, Tanzania
M. Rweyemamu
SACIDS Foundation for One Health, Africa Centre of Excellence P. O. Box 3297, Morogoro, Tanzania
F. Maree
Clinomics, Uitzich Road, Bainsvlei, Bloemfontein, 9301, Free State, South Africa
D. King
FAO World Reference Laboratory for FMD, The Pirbright Institute, Ash Road, Pirbright, Woking, GU24 ONF, United Kingdom
E. Mjingo
Tanzania Wildlife Research Institute, P. O. Box 661, Arusha, Tanzania
J. Wanda
Tanzania Wildlife Research Institute, P. O. Box 661, Arusha, Tanzania
D. Mdetele
Directorate of Veterinary Service, P. O Box 2870, Dodoma, Tanzania.
C. Kasanga
Department of Microbiology, Parasitology and Biotechnology, College of Veterinary Medicine and Biomedical Sciences, Sokoine University of Agriculture, P. O Box 3019, Morogoro, Tanzania and SACIDS Foundation for One Health, Africa Centre of Excellence P. O. Box 3297, Morogoro, Tanzania
Abstract
Foot and Mouth Disease Virus (FMDV) causes a highly devastating Foot and Mouth Disease (FMD) to all cloven hoofed livestock and wildlife. FMDV circulates worldwide as seven antigenically distinct serotypes (O, A, SAT1-3, C, and Asia1), and African buffalo act as reservoir of SAT1-3 serotypes. It remains unclear whether the buffalo can also act as carrier of the eurasian serotypes O and A, which occur in parts of East Africa. The screening of FMDV natural infection in buffalo and cattle was done using Enzyme Linked Immunosorbent Assay (ELISA), and then characterized by Solid phase competitive ELISA (SPCE) for FMDV antibodies specific to serotype O and A. The FMDV RNA screening and typing was done with one-step RT-PCR using PAN primers and serotype specific primers respectively. Results showed evidence for FMDV types O and A in buffalo and provides an important contribution to the knowledge for FMDV epidemiology in order to improve future tailored FMD control strategies in Tanzania
