Fasciola hepatica:(35S-Methionine) radio-labelled study of excretory/secretory products during development.

Abstract

Newly excysted juvenile (NEJ, DO) Day old (D1); 14, 28, 42 and 56 days old (D14, D28, D42, and D56) Fasciola Hepatica were biosynthetically radio-labelled by culturing with 35S-Methionine in Vitro. The total secreted proteins were analysed by electrophoresis in the presence of Sodium Dodecyl Sulphate (SDS-PAGE). There were noticeable differences and similarities in the Excretory/Secretory Products (ES) of the various ages of flukes, and the ES products of DO fluke had the simplest protein profile with only two components. Immunoprecipitation with serum from infected rats faintly recognized a 26KDa antigen in the reduced form of ES products of D1 fluke (D1 ES products). This serum recognised a 38 KDa antigen in the reduced form of ES of D14, D28, D42 and D56 flukes, and a 57 KDa antigen in the non-reduced form of adult (D56 flukes, and a 57 KDa antigen in the non-reduced form of adult (D56) ES. These results extends our knowledge of age-specific components of F. Hepatica.


(Tropical Veterinarian: 2003 21(1): 30-37)