Article Sidebar
Published:
Jan 20, 2014DOI:
10.4314/tjpr.v12i6.8Keywords:
Article Details
Submission of a manuscript to this journal is a representation that the manuscript has not been published previously and is not under consideration for publication elsewhere.
All authors named in each manuscript would be required to sign a form (to be supplied by the Editor) so that they may retain their copyright in the article but to assign to us (the Publishers) and its licensees in perpetuity, in all forms, formats and media (whether known or created in the future) to (i) publish, reproduce, distribute, display and store the contribution, (ii) translate the contribution into other languages, create adaptations, reprints, include within collections and create summaries, extracts and/or abstracts of the contribution, (iii) create any other derivative works(s) based on the contribution, (iv) to exploit all subsidiary rights in the contribution, (v) the inclusion of electronic links from the contribution to third party material where-ever it may be located, and (vi) license any thrid party to do any or all of the above.
Main Article Content
Anti-inflammatory Effects of Magnolia sieboldii Extract in Lipopolysaccharide-Stimulated RAW264.7 Macrophages
Abstract
Purpose: To investigate the effect of Magnolia sieboldii extract (MSE) on the production of proinflammatory cytokines by macrophage.
Methods: The whole plant of M. sieboldii was extracted with methanol at room temperature. The in vitro anti-inflammatory activity of MSE was investigated on lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. LPS-induced nitric oxide(NO) production was determined by Griess method. Production of pro-inflammatory cytokines including Interleukin-1 beta (IL-1 β), Interleukin-6 (IL-6), tumor necrosis factor- alpha (TNF-α) and cyclooxygenase-2 (COX-2) was examined using reverse trancriptase - polymerase chain reaction (RT-PCR) and Western blot analysis.
Results: Under in vitro conditions, MSE in doses ranging from 25 - 100 μg/mL significantly inhibited lipopolysaccharide (0.5 μg/mL)-induced nitric oxide production (p < 0.001), and the production of proinflammatory mediators (p < 0.05).
Conclusions: The anti-inflammatory effect of MSE on pro-inflammatory cytokines seems to ameliorate inflammatory symptoms via immune regulation.
Keywords: Anti-inflammatory, Nitric oxide, Cytokines, Immune regulation, Macrophage, Lipopolysaccharide, Polymyxin B, Magnolia sieboldii
