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Published:
Jun 26, 2013Keywords:
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Main Article Content
Suaeda japonica Makino Attenuates Lipopolysaccharide- Induced Neuro-Inflammatory Responses in BV-2 Microglia via NF-kappa B Signaling
Abstract
Purpose: To evaluate in vitro anti-oxidant and anti-neuro-inflammatory activities of Suaeda japonica extract (SJE) in lipopolysaccharide (LPS)-stimulated BV-2 microglial cells.
Methods: 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay was used to study the antioxidant effects. 3-(4, 5-dimethylthiazol-2-yl)-2, 5- diphenyl-tetrazolium bromide (MTT) assay was used to study cell viabilities. LPS-stimulated BV- microglial cells were used to study the gene
expression and production of inflammatory mediators determined by Western blot analysis.
Results: SJE significantly inhibited the DPPH generated free radicals, and suppressed LPS-induced expression of inducible nitric oxide synthase (iNOS) and production of nitric oxide (NO) in a concentration-dependent manner. It decreased LPS-induced expression of some inflammatory
mediators and pro-inflammatory cytokines (cyclooxygenase-2 and interleukin (IL)-6). This suppression of inflammatory mediators was nuclear factor kappa-B (NF-êB)-dependent.
Conclusion: Our findings imply that SJE may be a potential therapeutic agent in regulating microgliamediated neuroinflammatory responses observed in several neurodegenerative diseases.
Keywords: Suaeda japonica, Antioxidant activity, Anti-inflammatory activity, Microglial Cells, iNOS, IL-6.
