Article Sidebar
Published:
May 9, 2013DOI:
10.4314/tjpr.v12i2.2Keywords:
Article Details
Submission of a manuscript to this journal is a representation that the manuscript has not been published previously and is not under consideration for publication elsewhere.
All authors named in each manuscript would be required to sign a form (to be supplied by the Editor) so that they may retain their copyright in the article but to assign to us (the Publishers) and its licensees in perpetuity, in all forms, formats and media (whether known or created in the future) to (i) publish, reproduce, distribute, display and store the contribution, (ii) translate the contribution into other languages, create adaptations, reprints, include within collections and create summaries, extracts and/or abstracts of the contribution, (iii) create any other derivative works(s) based on the contribution, (iv) to exploit all subsidiary rights in the contribution, (v) the inclusion of electronic links from the contribution to third party material where-ever it may be located, and (vi) license any thrid party to do any or all of the above.
Main Article Content
In vivo Evaluation of Amoxicillin Trihydrate and Clarithromycin-Loaded Mucoadhesive Microspheres for H. pylori Eradication
Abstract
Purpose: To evaluate in vivo H. pylori clearance efficacy of formulated mucoadhesive microspheres of amoxicillin trihydrate and clarithromycin.
Methods: Amoxicillin trihydrate and clarithromycin mucoadhesive microspheres were prepared by solvent evaporation method using Carbopol 974P, HPMC K4M and Eudragit RS 100. In vivo clearance efficacy of the microspheres was evaluated in a Wistar rat model after induction of H. pylori infection. Amoxicillin and clarithromycin-loaded microspheres were administered twice daily for three days. H. Pylori clearance was evaluated by assessing colony count.
Results: Treatment with plain drug solution (90 mg/kg amoxicillin and 45 mg/kg clarithromycin) resulted in a colony count of log 1.25 ± 0.56 CFU and clearance rate of 60 %, while mucoadhesive microspheres-loaded dose of 45 mg/kg amoxicillin and 22.5 mg/kg clarithromycin resulted in complete (100 %) eradication of H. pylori infection.
Conclusion: The developed mucoadhesive amoxicillin/clarithromycin microspheres can potentially be used to effectively eradicate H. pylori infection.
Keywords: Amoxicillin, Carbopol, Clarithromycin, H. pylori, Hydroxypropyl methylcellulose, Microspheres, Mucoadhesive
