Article Sidebar
Published:
Mar 7, 2013Keywords:
Article Details
Submission of a manuscript to this journal is a representation that the manuscript has not been published previously and is not under consideration for publication elsewhere.
All authors named in each manuscript would be required to sign a form (to be supplied by the Editor) so that they may retain their copyright in the article but to assign to us (the Publishers) and its licensees in perpetuity, in all forms, formats and media (whether known or created in the future) to (i) publish, reproduce, distribute, display and store the contribution, (ii) translate the contribution into other languages, create adaptations, reprints, include within collections and create summaries, extracts and/or abstracts of the contribution, (iii) create any other derivative works(s) based on the contribution, (iv) to exploit all subsidiary rights in the contribution, (v) the inclusion of electronic links from the contribution to third party material where-ever it may be located, and (vi) license any thrid party to do any or all of the above.
Main Article Content
Phytochemical Screening and Cytotoxicity of Crude Extracts of Vatica diospyroides Symington Type LS
Abstract
Purpose: To investigate the phytochemistry and cytotoxic activity of the leaf, branch and fruit extracts of Vatica diospyroides Symington type LS, an endangered medicinal plant in the peninsula of Thailand.
Methods: Extracts of the mature leaves, branches and fruit (both cotyledon and pericarp) of five-year old tree were obtained using various organic solvents. Thin–layer chromatography (TLC) and phytochemical screening were employed to identify the chemical constituents. Cytotoxicity was
characterized by 50 % inhibition (IC50) of human breast cancer cell lines (MCF-7 and MDA-MB-468) using 3-(4,5-dimethylthaizol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
Results: The extracts of the plant parts differed in chemical composition. Terpenoids and anthraquinones were present in all extracts, while cardiac glycosides and tannins were isolated from the branches and leaves, respectively. The acetone and methanol extracts of the fruit cotyledons were highly cytotoxic against MDA-MB-468 cell line (IC50 = 3.1 ìg/ml for both extracts), whereas acetone and dichloromethane extracts of the fruit pericarps were highly active against MCF-7 cell line (IC50 = 18.2 and 21.0 ìg/ml, respectively). The other extracts were less active.
Conclusion: This work revealed the potentials of V. diospyroides fruit as a cytotoxic agent against human breast cancer cell lines. However, further studies, including identification and purification of the active compounds, will need to be pursued.
Keywords: Breast cancer, Crude extract, Dipterocarpaceae, MCF-7, MDA-MB-468, Vatica diospyroides
