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Published:
Feb 25, 2013DOI:
10.4314/tjpr.v11i6.4Keywords:
Article Details
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Main Article Content
Inhibition of Pro-inflammatory Cytokines by Ethyl Acetate Extract of Scrophularia striata
Abstract
Purpose: To investigate the effect of ethyl acetate extract of S. striata on pro-inflammatory production by macrophages.
Methods: Mouse peritoneal macrophages were cultured in solvent either alone or with 2 ìg/ml lipopolysaccaride (LPS) with/without different doses of ethyl acetate extract of S. striata. Production of pro-inflammatory cytokines including interleukin 1â (IL-1â), tumor necrosis factor - á (TNF-á) and
prostaglandin E2 (PGE2) were examined using ELISA.
Results: Ethyl acetate fraction of S. striata in doses of 10, 50, 100 and 200 ìg/ml significantly (p < 0.05) inhibited pro-inflammatory mediators (IL-1â, TNF-á, and PGE2) production by LPS stimulated peritoneal macrophages.
Conclusion: The anti-inflammatory effect of the extract on pro-inflammatory cytokines may ameliorate inflammatory diseases, possibly via an immunomodulatory mechanism.
Keywords: Pro-inflammatory, Cytokines, Ethyl acetate extract, Scrophularia striata
