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Cisplatin Induces Up-Regulation of KAI1, a Metastasis Suppressor Gene, in MCF-7 Breast Cancer Cell Line
Abstract
Purpose: To investigate the effect of cisplatin on cell toxicity and metastasis through modulation of KAI1 gene expression.
Methods: MCF-7cells were incubated with different concentrations of cisplatin for 24 h. RNA was extracted by trizol and cDNA synthesized. KAI1 and TBP were chosen as target and internal control genes, respectively. Specific primers were designed by primer express software, v.3.0. KAI1/TBP and gene expression ratio was calculated using the formula, 2 -ΔΔCt.
Results: Cisplatin exerted a dose-dependent inhibitory effect on the viability of highly metastatic MCF-7 cells. KAI1/TBP gene expression ratios were 1.97 ± 0.19 (p < 0.05), 2.96 ± 0.55 (p < 0.05), 9.06 ± 0.27 (p < 0.001) and 12.38 ± 0.88 (p < 0.01) in 10, 20, 50 and 100 μM concentrations of cisplatin. Conclusion: These findings indicate that cisplatin can inhibit metastasis by up-regulating KAI1 gene in MCF-7cells.
Keywords: Cisplatin; KAI1; Metastasis; Breast Cancer; Real-time PCR.
