Article Sidebar
Article Details
Submission of a manuscript to this journal is a representation that the manuscript has not been published previously and is not under consideration for publication elsewhere.
All authors named in each manuscript would be required to sign a form (to be supplied by the Editor) so that they may retain their copyright in the article but to assign to us (the Publishers) and its licensees in perpetuity, in all forms, formats and media (whether known or created in the future) to (i) publish, reproduce, distribute, display and store the contribution, (ii) translate the contribution into other languages, create adaptations, reprints, include within collections and create summaries, extracts and/or abstracts of the contribution, (iii) create any other derivative works(s) based on the contribution, (iv) to exploit all subsidiary rights in the contribution, (v) the inclusion of electronic links from the contribution to third party material where-ever it may be located, and (vi) license any thrid party to do any or all of the above.
Main Article Content
Effect of Process Factors on the Properties of Doxycycline Nanovesicles
Abstract
Purpose: To develop and evaluation ascorbyl palmitate niosomes in order to achieve a transdermal and/or systemic nanocarier of doxycycline.
Methods: Vesicles were formed from ascorbyl palmitate in combination with cholesterol and a negatively charged lipid, dicetyl phosphate. Niosomes were prepared by film hydration method followed by sonication in which aqueous doxycycline solution (in phosphate buffered saline) was encapsulated in the aqueous regions of the vesicles. The vesicles were evaluated for entrapment and in vitro release as well as for their thermal properties and shape by ultraviolet spectroscopy (UV), differential scanning calorimetry (DSC) and scanning electron microscopy (SEM), respectively. The effect of process conditions - sonication time, pH, hydration temperature and centrifuge speed - on niosome properties was investigated.
Results: DSC of pure lipids, vesicles dispersion and mixture of lipids confirmed the formation of niosomes. Other results show that > 90 % of drug was entrapped in the vesicles and the vesicles were spherical in shape. Drug release from the vesicles was slow (< 60 % after 8 h). Nanovesicle size was significantly (p < 0.05) affected by sonication time and hydration pH. Although hydration temperature of 100 °C promoted the production of smaller vesicles, this temperature would likely cause drug degradation.
Conclusion: The use of ascorbyl palmitate along with cholesterol and a charge inducer (dicetyl phosphate) yielded vesicles that satisfactorily encapsulated doxycycline solution. The resulting system can be applied for the formulation of doxycycline niosomes.
Keywords: Doxycicline, Vesicles, Drug delivery, Ascorbyl palmitate, Niosomes.
