Article Sidebar
DOI:
10.4314/tjpr.v10i5.6Keywords:
Article Details
Submission of a manuscript to this journal is a representation that the manuscript has not been published previously and is not under consideration for publication elsewhere.
All authors named in each manuscript would be required to sign a form (to be supplied by the Editor) so that they may retain their copyright in the article but to assign to us (the Publishers) and its licensees in perpetuity, in all forms, formats and media (whether known or created in the future) to (i) publish, reproduce, distribute, display and store the contribution, (ii) translate the contribution into other languages, create adaptations, reprints, include within collections and create summaries, extracts and/or abstracts of the contribution, (iii) create any other derivative works(s) based on the contribution, (iv) to exploit all subsidiary rights in the contribution, (v) the inclusion of electronic links from the contribution to third party material where-ever it may be located, and (vi) license any thrid party to do any or all of the above.
Main Article Content
Candidacidal Action of CF66I, an Antifungal Compound Produced by Burkholderia Cepacia
Abstract
Purpose: To investigate the candidacidal activity of a novel compound CF66I and attempt to elucidate its mode of action.
Methods: Minimal inhibition concentrations (MIC) and minimal fungicidal concentrations (MFC) of CF66I for Candida albicans were determined using serial broth dilution method. Colony count assay, K+ release, propidium iodide (PI) uptake and electron microscopy were used to determine the manner in which CF66I exerts its candidacidal effects.
Results: MIC and MFC of CF66I were determined to be 5.0 - 7.0 μg/ml and 14.0 - 18.0 μg/ml. It showed strong candidacidal activity over a wide pH range (4.0-7.4). The compound killed C. albicans within 30 s. It preferentially bound to the surface of C. albicans via a specific interaction with β-1,6-glucan, one of fungal cell wall components, and then affected the normal structure of fungal cell wall and induced cell wall thickening, which was confirmed by transmission electron microscopy (TEM) CF66I also exerted its candidacidal activity against cell membrane, eventually induced K+ release from C. albicans and PI uptake. Membrane-attacking ability was also found via electron microscopy.
Conclusion: These results suggest that CF66I exerted its candidacidal activity on multiple target sites on C. albicans, and may have considerable potential for development of a new class of antifungal agents.
Keywords: Burkholderia cepacia, Candidacidal activity; CF66I; Candida albicans; Mode of action
