Article Sidebar
Keywords:
Article Details
Submission of a manuscript to this journal is a representation that the manuscript has not been published previously and is not under consideration for publication elsewhere.
All authors named in each manuscript would be required to sign a form (to be supplied by the Editor) so that they may retain their copyright in the article but to assign to us (the Publishers) and its licensees in perpetuity, in all forms, formats and media (whether known or created in the future) to (i) publish, reproduce, distribute, display and store the contribution, (ii) translate the contribution into other languages, create adaptations, reprints, include within collections and create summaries, extracts and/or abstracts of the contribution, (iii) create any other derivative works(s) based on the contribution, (iv) to exploit all subsidiary rights in the contribution, (v) the inclusion of electronic links from the contribution to third party material where-ever it may be located, and (vi) license any thrid party to do any or all of the above.
Main Article Content
Simultaneous Analysis of Bioactive Markers from Orthosiphon Stamineus Benth Leaves Extracts by Reverse Phase High Performance Liquid Chromatography
Abstract
Purpose: To develop a reverse phase high performance liquid chromatography (RP-HPLC) method for the analysis of the crude extracts of Orthosiphon stamineus.
Methods: A simple and facile analytical method was developed using RP- HPLC with UV detection for the identification and quantitation of bioactive markers present in O. stamineus extracts. Four different bioactive markers were used for the analysis, namely, rosmarinic acid, orthosiphol-A, 3’-hydroxy-5, 6, 7,4’-tetramethoxyflavone (TMF) and 5, 6, 7, 3’, 4’-pentamethoxyflavone (sinensetin), using an isocratic mobile phase methanol: tetrahydrofuran: water (0.1% H3PO4) (55:5:40) on Nucleosil C-18 column (250 mm x 4.6 mm i.d., 5 ìm particle size) at a flow rate of 0.7 ml/min and detection at 330 nm with 30 min separation time.
Results: The bioactive marker orthosiphol A was identified and isolated from the water extract of O. stamineus leaves. The standard calibration curves for the marker were linear in the range 0.01 - 500 ìg/ml with a regression coefficient (r2) > 0.9996. The recoveries of the four markers were in the range 83.2 to 106.4 % at relative standard deviation (RSD) values < 5 %. The limit of detection (LOD) and of quantification (LOQ) were 2 and 20 ng/ml, respectively.
Conclusion: The developed method is simple, sensitive and specific for simultaneous determination of the indicated marker compounds either qualitatively or quantitatively, and may be used as a fingerprint
profile for the standardization of extractives or herbal medicines from O. stamineus.
