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Feb 11, 2025Keywords:
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Main Article Content
In vivo investigation of Roselle flower (Hibiscus sabdariffa L.) and Bee pollen (Tetragonula laeviceps) on carbon tetrachloride-induced spermatozoa damage
Abstract
Purpose: To determine the effect of roselle flower and bee pollen on carbon tetrachloride (CCl4)-induced spermatozoa damage. Methods: Roselle flowers (Hibiscus sabdariffa L.) and bee pollen (Tetragonula laeviceps) were obtained from Bali, Indonesia. A total of 24 BALB/c (Mus musculus) mice were used and randomly assigned into control, negative control (CCl4-induced), positive control (CCl4 + ascorbic acid) and treatment groups (25, 50, and 100 mg/20 g). Spermatozoa-induced damage was done using carbon tetrachloride (CCl4). Sperm levels, movement, viability, and morphology of the spermatozoa were evaluated. Results: Roselle and bee pollen granules at 50 mg/20 g (11.2 mg of roselle extract and 28 mg of pollen extract), demonstrated the most significant improvement in spermatozoa levels, movement, viability, and morphological characteristics compared to negative controls (p < 0.05). Conclusion: Roselle and bee pollen granular combination offers a promising natural intervention for mitigating chemically induced reproductive toxicity. It suggests potential therapeutic applications in preserving male reproductive health and counteracting oxidative stress-related spermatogenic damage.
