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Feb 11, 2025Keywords:
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Main Article Content
Anti-inflammatory activity of Cinnamomum cassia bark on macrophage migration inhibitory factor (MIF) protein and expression levels of pro-inflammatory mRNAs and cytokines
Abstract
Purpose: To assess the inhibitory effect of extract of Cinnamomum cassia (cinnamon) bark on
macrophage migration inhibitory factor (MIF) protein, expression levels of pro-inflammatory mRNAs and
cytokines of macrophage RAW 264.7 cells.
Methods: Inhibition of MIF tautomerase activity following administration of cinnamon bark extract was
measured and the IC50 value was determined. Expressions of pro-inflammatory TGF-β1, TNF-α and IL-
8 mRNAs were determined using real-time quantitative reverse transcription polymerase chain reaction
(qRT-PCR). Also, IL-1β and IFN-γ cytokines were evaluated using enzyme-linked immunosorbent assay
(ELISA).
Results: Cinnamon bark extract displayed inhibitory activity on MIF protein with an IC50 value lower
than 1 mg/L, and reduction of the expressed levels of TGF-β1, TNF-α and IL-8 mRNAs; and IL-1β, and
IFN-γ cytokines of macrophage RAW 264.7 cells with varying levels of effectiveness depending on the
specific pro-inflammatory mediators.
Conclusion: Extract of cinnamon bark decreases the expression of TGF-β1, TNF-α, and IL-8 proinflammatory
mRNAs, as well as IL-1β and IFN-γ pro-inflammatory cytokines released by macrophage
RAW 264.7 cells induced by LPS.
