Article Sidebar
Published:
Feb 5, 2024Keywords:
Article Details
Submission of a manuscript to this journal is a representation that the manuscript has not been published previously and is not under consideration for publication elsewhere.
All authors named in each manuscript would be required to sign a form (to be supplied by the Editor) so that they may retain their copyright in the article but to assign to us (the Publishers) and its licensees in perpetuity, in all forms, formats and media (whether known or created in the future) to (i) publish, reproduce, distribute, display and store the contribution, (ii) translate the contribution into other languages, create adaptations, reprints, include within collections and create summaries, extracts and/or abstracts of the contribution, (iii) create any other derivative works(s) based on the contribution, (iv) to exploit all subsidiary rights in the contribution, (v) the inclusion of electronic links from the contribution to third party material where-ever it may be located, and (vi) license any thrid party to do any or all of the above.
Main Article Content
Cytotoxicity of luteolin, a flavonoid compound isolated from Anthemis palestina
Abstract
Purpose: To determine the active principle responsible for the cytotoxic effect of Anthemis palestina (Reut. ex Kotschy) Reut. ex Boiss. (Asteraceae).
Methods: A bioassay-guided fractionation was used to isolate the active principle, luteolin, which structure was elucidated using 1H and 13C NMR. The cytotoxic effects of luteolin and doxorubicin (positive control) in the breast cancer cell lines MDA-MB-231 and MCF-7, and in normal fibroblasts, were determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay.
Results: Luteolin was isolated from the ethyl acetate extract of the aerial parts of Anthemis palestina. With an IC50 value of 14.91 ± 5.77 µM for MDA-MB-231 cells, luteolin was less active than doxorubicin. However, with respect to MCF-7 cells, there was no significant difference in the cytotoxicity values of luteolin and doxorubicin, with IC50 value of 29.28 ± 11.85 µM for luteolin. However, with an IC50 value of 51.39 ± 18.51 µM against fibroblasts, luteolin was significantly safer than doxorubicin.
Conclusion: Luteolin might be responsible for the cytotoxicity of Anthemis palestina. The high level of luteolin cytotoxicity indicates the potential benefits of Anthemis palestina, not only in terms of its taste, but also for its likely positive therapeutic impact on cancer, especially breast cancer.
