Article Sidebar
Article Details
Submission of a manuscript to this journal is a representation that the manuscript has not been published previously and is not under consideration for publication elsewhere.
All authors named in each manuscript would be required to sign a form (to be supplied by the Editor) so that they may retain their copyright in the article but to assign to us (the Publishers) and its licensees in perpetuity, in all forms, formats and media (whether known or created in the future) to (i) publish, reproduce, distribute, display and store the contribution, (ii) translate the contribution into other languages, create adaptations, reprints, include within collections and create summaries, extracts and/or abstracts of the contribution, (iii) create any other derivative works(s) based on the contribution, (iv) to exploit all subsidiary rights in the contribution, (v) the inclusion of electronic links from the contribution to third party material where-ever it may be located, and (vi) license any thrid party to do any or all of the above.
Main Article Content
Antioxidant and anti-inflammatory effects of Aegle marmelos fruit and Moringa oleifera leaf extracts on lipopolysaccharide-stimulated BV2 microglial cells
Abstract
Purpose: To determine the antioxidant and anti-inflammatory effects of Aegle marmelos fruit and Moringa oleifera (M. oleifera) Lam leaf extracts on LPS-stimulated BV2 microglial cells.
Methods: BV2 cells were incubated with LPS for 24 h in the presence or absence of A. marmelos fruit extract or M. oleifera leaf extract. Subsequently, the levels of reactive oxygen species (ROS), nitric oxide (NO), tumor necrosis factor α (TNF α), and interleukins (IL) 6 were assessed. Thereafter, 2,2-diphenyl-1-picrylhydraz (DPPH), 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), and ferric reducing antioxidant power (FRAP) antioxidant assays were performed.
Results: The levels of ROS, NO, TNF α, and IL 6 in LPS-treated BV2 cells were significantly higher when compared to those in control group (p < 0.05). However, exposure of LPS-treated BV2 cells to either A. marmelos or M. oleifera leaf extract led to significant decrease in the levels of ROS and inflammatory mediators, when compared with LPS-treated group (p < 0.05). Decrease in the levels of ROS and inflammatory mediators in M. oleifera leaf extract-treated cells was also significantly larger when compared with A. marmelos-treated group (p < 0.05). The DPPH, ABTS, and FRAP assays revealed that M. oleifera leaf extracts had greater antioxidant capacity than A. marmelos extracts.
Conclusion: Aegle marmelos fruit and M. oleifera leaf extracts exert antioxidant and anti-inflammatory effects on LPS activated BV2 cells in an in vitro model. Therefore, A. marmelos fruit and M. oleifera leaf extracts may be agents in the development of novel anti oxidative and anti-neuroinflammatory herbal medicines, or as food supplements.
