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MiR-33a-5p negatively regulates ovariectomy-induced osteoporosis in rats by decreasing WNT10B
Abstract
Purpose: To investigate the role and potential mechanism of action of microribonucleic acid (miR)-33-5p in osteoporosis after ovariectomy.
Methods: A postmenopausal osteoporosis model was established using an ovariectomized (OVX) rat. Primary bone marrow-derived mesenchymal stem cells (BMMSCs) were obtained from female rats, and were transfected with mimics or inhibitors. Reverse transcription-polymerase chain reaction (RT-PCR) and western blotting were preformed to determine the level of miR-33a-5p and target proteins, while luciferase reporter assay was used to verify whether WNT10B is a target of miR-33a-5p. Microcomputed tomography (micro-CT) analysis was employed to evaluate bone formation.
Results: Bone mineral density (BMD), determined by micro-CT, was significantly decreased in OVX group. MiR-33a-5p was in negative correlation with WNT10B after surgery, and WNT10B was found to be a target of miR-33a-5p. Additionally, inhibition of miR-33a-5p facilitated osteogenic differentiation in vitro, and promoted bone formation in vivo.
Conclusion: Inhibition of miR-33a-5p accelerates bone formation by increasing WNT10B levels. Thus, miR-33a-5p is a potential target for the treatment of osteoporosis.
