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Published:
Feb 18, 2023Keywords:
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Main Article Content
Marsilea crenata extract modulates SOD1 expression in male rat testes via Nrf2 pathway
Abstract
Purpose: To investigate the potential effect of M. crenata ethanol extract as source of antioxidants through SOD1 and Nrf2 expression levels in rat testes.
Methods: Phytochemical analysis of M. crenata was performed using high-performance liquid chromatography (HPLC), while antioxidant activity was determined by 2.2-diphenyl-1-picrylhydrazyl (DPPH radical) assay. This study used 24 male rats (n = 6), divided into 4 groups: control (normal rat without any treatment); PS1, PS2, and PS3 (normal rat which orally received ethanol extract of M. crenata leaves at a dose of 43.2, 86.4, and 129.6 mg/200 g body weight for 30 days, respectively). The expression levels of Nrf2 and SOD1 were analyzed by flow cytometry.
Results: Quercetin and genistein were found in ethanol extract of M. crenata leaves with retention times of 8.812 and 22.309 min, respectively. M. crenata leaves extract was categorized as a strong antioxidant (IC50: 33.68 ppm). There was an increase in Nrf2 expression in rat testis in PS1 group. An upregulation of SOD1 expression was found in PS2 group.
Conclusion: M. crenata extract is a rich natural source of antioxidants and phytoestrogen and thus may be useful in the management of testicular dysfunctions. More studies will be required to firmly establish this finding.
