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Antioxidant, antidiabetic and cytotoxicity effects of Euadenia trifoliata leaves extracts
Abstract
Purpose: Euadenia trifoliata plant is used traditionally for the management and treatment of several diseases. The current study investigates in vitro antidiabetic, antioxidant and cytotoxic potential of the plant.
Methods: The antioxidant activity of the hexane, ethylacetate, methanol and water extracts of Euadenia trifoliata was determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2-azino-bis(3-ethylbenzothiazoline-6)-sulphonic acid (ABTS), iron chelation, hydroxyl and nitric oxide radical scavenging capability assays. The antidiabetic activity was evaluated by determining the inhibitory effect of the extracts on the activities of α-amylase and α-glucosidase enzyme. Cytotoxic activity was determined against brine shrimps and MCF-7 cancer cell lines using brine shrimp lethality and MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assays respectively.
Results: Methanol and ethyl acetate extracts showed significant (p ≤ 0.05) antidiabetic activity against α-amylase equally (1.38mg/mL) and α-glucosidase (0.52mg/mL and 0.70 mg/mL) respectively compared to hexane and aqueous extracts. Also, both methanol and ethylacetate extracts showed better antioxidant activity compared to other solvent extracts. The ethylacetate extract was found to be highly cytotoxic and aqueous extract showed no cytotoxicity against brine shrimps. None of the extracts were found to be cytotoxic against MCF-7 breast cancer.
Conclusion: Methanol and ethylacetate extracts of E. trifoliata possess better antioxidant, antidiabetics and cytotoxic activities against brine shrimps compared to non-polar (hexane) and polar (aqueous) extracts.
