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Effect of bone marrow mesenchymal stem cells on tissue reconstruction and angiogenesis following fat transplantation
Abstract
Purpose: To investigate the effect of bone marrow mesenchymal stem cells (BMSCs) on tissue reconstruction and microvascular formation after fat transplantation.
Methods: Adipose tissue from male BALB/C nude mice were used to extract and culture BMSCs. Eighty mice were assigned to control and study groups. Control mice were injected with 0.3 g adipose tissue in normal saline, while the study group was injected with 0.3 g adipose tissue containing 5 × 105 mouse BMSCs. Micro-vessel count was determined by CD31 antibody staining, while VEGF protein expression was assayed by Western blotting.
Results: At each time point, the micro-vessel count of adipose tissue was significantly higher in the study group than in controls (p < 0.05). At 12 weeks, VEGF protein in adipose tissue of mice was significantly higher in the study mice than in control mice (p < 0.05).
Conclusion: BMSCs enhance adipose tissue reconstruction and induce adipose tissue microvascular formation through up-regulation of the protein expression of VEGF.
