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Ophiopogonin B suppresses the growth and epithelialmesenchymal transition in laryngeal cancer cells by inhibiting FAK/AKT signaling pathway
Abstract
Purpose: To investigate the effect of ophiopogonin B on laryngeal cancer cells, and whether it is related to epithelial mesenchymal transition (EMT).
Methods: Human laryngeal cancer cells (AMC-HN-8) were used as tool cells in vitro. Cell growth was characterized by cell viability and proliferation while cell apoptosis was analyzed using Annexin V/PI staining. Cell invasion and migration were assessed Transwell assay.
Results: Ophiopogonin B inhibited the viability and proliferation of AMC-HN-8 cells at a concentration > 10 μM. Cell apoptosis was enhanced after treatment with ophiopogonin B. The fraction of apoptotic cells for 5, 10 and 20 μM groups were 6.25, 16.16, 28.3 and 39 %, respectively. Transwell assay data showed that ophiopogonin B inhibited the invasion and migration of laryngeal cancer cells. In addition, the expression of N-cad and snail (EMT inducer) was inhibited, while the expression of E-cad was enhanced. These results also indicate that ophiopogonin B inhibited the migration and EMT of laryngeal cancer cells. Besides, ophiopogonin B inhibited the phosphorylation of FAK and AKT.
Conclusion: These results indicate that ophiopogonin B suppresses the growth, migration and EMT in laryngeal cancer cell by inhibiting FAK/AKT signaling pathway. These results provide some ideas for improved treatment of laryngeal cancer.
