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Published:
Feb 17, 2022Keywords:
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Main Article Content
Erdosteine prevents contrast-induced renal oxidative stress damage in mice
Abstract
Purpose: To investigate the protective effect of erdosteine on contrast-induced renal oxidative stress in mice.
Methods: C57BL/6 mice were injected intraperitoneally with contrast medium to establish an acute kidney injury model (AKI). Renal function, blood urea nitrogen (BUN) and serum creatinine (SCr) were determined. Also, oxidative stress, lactate dehydrogenase (LDH), malondialdehyde (MDA), superoxide dismutase (SOD) and reduced glutathione (GSH) were evaluated. And the renal tissue structure was examined by light microscopy, while Western blot (WB) and Real-time polymerase chain reaction (RT-PCR) were used to determine the expressions of senescence-related factor, Nrf-2 and downstream antioxidant factor.
Results: Erdosteine improved the renal structure of mice and significantly decreased serum BUN and SCr levels. In addition, erdosteine promoted the expression of antioxidant enzymes SOD1, SOD2, GPX1 and GPX3 in renal tissues, decreased the content of ROS, and inhibited the content of LDH and MDA in serum. Also, WB and RT-PCR results showed that erdosteine activated Nrf2 pathway, thereby alleviating contrast-induced renal injury.
Conclusion: Erdosteine inhibits contrast-induced renal oxidative stress in mice and delays cell senescence by activating Nrf2 pathway. This will be of great significance in the treatment of contrast-induced nephropathy.
