Article Sidebar
Published:
Jan 24, 2022Keywords:
Article Details
Submission of a manuscript to this journal is a representation that the manuscript has not been published previously and is not under consideration for publication elsewhere.
All authors named in each manuscript would be required to sign a form (to be supplied by the Editor) so that they may retain their copyright in the article but to assign to us (the Publishers) and its licensees in perpetuity, in all forms, formats and media (whether known or created in the future) to (i) publish, reproduce, distribute, display and store the contribution, (ii) translate the contribution into other languages, create adaptations, reprints, include within collections and create summaries, extracts and/or abstracts of the contribution, (iii) create any other derivative works(s) based on the contribution, (iv) to exploit all subsidiary rights in the contribution, (v) the inclusion of electronic links from the contribution to third party material where-ever it may be located, and (vi) license any thrid party to do any or all of the above.
Main Article Content
Antimycobacterial activity of aqueous and methanol extracts of nine plants against Mycobacterium bacteria
Abstract
Purpose: The present study was done to evaluate the antimycobacterial activity of aqueous and methanol extracts of nine plants viz., Buddleja saligna, Carpobrotus dimidiatus, Capparis tomentosa, Dichrostachys cinerea, Ekebergia capensis, Ficus sur, Gunnera perpensa, Leonotis leonurus and Tetradenia riparia in South Africa.
Methods: Aqueous and methanol extracts of the leaves the plants were tested in vitro for their activity against Mycobacterium smegmatis, Mycobacterium tuberculosis H37Rv (ATCC 25177) and three well-characterized clinical isolates of MDR and XDR-TB isolates using the agar incorporation method. The minimum inhibitory concentration (MIC) of each of the active plant extract was determined using the broth microdilution method. Cytotoxic effect was evaluated against the mouse BALB/C monocyte macrophage (J774.2) and peripheral blood mononuclear cells (PBMCs) whole the toxicity screening was done using the brine shrimp lethality assay. Composition of each of the plants was determined using thin layer chromatography while qualitative analysis of antimycobacterial compounds was done using TLC-Bioautography
Results: The methanol extracts of B. saligna, C. tormentosa and C. dimidiatus; and aqueous extracts of G. perpensa and T. riparia possessed significant activity against M. smegmatis, M. tuberculosis H37Rv (ATCC 25177) and the three well-characterized clinical isolates of MDR and XDR-TB. Except for a high concentration of G. perpensa none of the other plants which possessed antimycobacterial activity showed any toxic or cytotoxic activity.
Conclusion: Our findings show that B. saligna, C. tormentosa, C. dimidiatus, G. perpensa, and T. riparia have antimycobacterial activity. Further studies would aim at isolation and identification of the active compounds from the plants extracts which had positive antimycobacterial activity.
