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Long intergenic non-protein coding RNA 00174 promotes cardiac hypertrophy by targeting miR-150-5p
Abstract
Purpose: miR-150-5p is associated with several diseases but its relation with cardiac hypertrophy (CH) is yet to be unveiled. However, long intergenic non-protein coding RNA 174 (LINC00174) is implicated in CH. The purpose of this study was to investigate the roles of LINC00174 and miR-150-5p in relation to CH progression.
Methods: A mechanical cyclic stretch was applied to create 4 groups of H9 cardiomyocytes: 15 % 7, 15, 24 and 48 hr stretches, as well as unstretched control. Transfection method was used to up-regulate expression levels of LINC00174 and miR-150-5p in H9 cardiomyocytes. RT-qPCR and Western blot were used to determine the effect of LINC00174 on mRNA expression levels of miR-150-5p, proliferation and apoptosis biomarkers. Cell proliferation was determined by CCK-8 assay and the target gene of LINC00174 was detected by luciferase reporter assay.
Results: Expression level of LINC00174 was high in H9 stretched cardiomyocytes. When overexpressed, it enhanced the mRNA expression of proliferation biomarkers and down-regulated apoptosis biomarkers. More so, miR-150-5p was down-regulated in H9 stretched cardiomyocytes and it was a direct target of LINC00174. Furthermore, miR-1505p restoration reversed the effects of LINC00174 overexpression on proliferation and apoptosis biomarkers.
Conclusion: LINC00174 and miR-150-5p may be novel biomarkers for early diagnosis of CH.
