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Studies on the expression and biological functions of ZIC5 in hepatocellular carcinoma
Abstract
Purpose: To study the expression of zinc finger protein of the cerebellum 5 (ZIC5) and its biological functions in hepatocellular carcinoma (HCC).
Methods: Sixty-five patients undergoing HCC surgery were selected. Expression of ZIC5 in HCC and para-carcinoma tissue was examined by quantitative real time-polymerase chain reaction (qRT-PCR) and western blotting. The relationship between ZIC5 expression and clinicopathological features, postoperative survival rate, and prognosis of liver cancer patients was analyzed by t-test, Kaplan-Meier method, and Cox regression analysis, respectively. The effects of ZIC5 silencing on Huh-7 cell proliferation, migration, invasion, and apoptosis were assessed using Cell Counting Kit-8 (CCK-8), wound healing assay, Transwell assay, and flow cytometry, respectively.
Results: ZIC5 expression in liver cancer tissue was significantly higher than in the para-carcinoma tissue and was significantly correlated with TNM stage and differentiation degree (p < 0.001). The overall survival rate of patients with high ZIC5 expression level was significantly lower than that of patients with low ZIC5 expression (p < 0.01). ZIC5 expression, TNM stage, and differentiation degree were independent prognostic factors. ZIC5 silencing significantly inhibited the proliferative, migratory, invasive, and anti-apoptotic capacity of Huh-7 cells (p < 0.01).
Conclusion: ZIC5 is highly expressed in HCC, and this can promote liver cancer cell proliferation, migration, and invasion.
