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Mediator complex subunit 19 regulates the proliferation, migration and invasion of human breast cancer cells
Abstract
Purpose: To investigate the therapeutic implication of mediator complex subunit 19 (Med19) in breast cancer cells.
Methods: The mRNA expression of Med19 was assayed using qRT-PCR. Cell viability was determined with 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyl tetrazolium bromide (MTT) assay, while 4′,6-diamidino-2- phenylindole (DAPI) and annexin V/propidium iodide (PI) assays were used for determination of apoptosis. Wound healing and Transwell assays were used for the determination of cell migration and invasion. Western blotting analysis was used for assay of protein expression levels.
Results: The results showed that Med19 was significantly (p < 0.05) upregulated in human breast cancer cell lines, relative to normal cells. The up-regulations ranged from 3.7-fold in UACC-2087 cells to 6.4-fold in BT-20 cells. Moreover, Med19 silencing caused significant decrease in the proliferation of BT-20 breast cancer cells (p < 0.05). The inhibition of cell proliferation was due to the induction of apoptosis, as was evident in increased Bax/Bcl-2 ratio. Annexin V/PI staining revealed 6 % apoptosis in si-NC-transfected, and about 13.30 % in si-Med19-transfected BT-20 cells. Wound healing and Transwell assays revealed that the invasion of BT-20 breast cancer cells significantly decreased upon Med19 silencing.
Conclusion: Med19 regulates the proliferation, migration and invasion of human breast cancer cells. Thus, Med19 may be beneficial in the treatment of breast cancer.
