Article Sidebar
Published:
Jul 22, 2021Keywords:
Article Details
Submission of a manuscript to this journal is a representation that the manuscript has not been published previously and is not under consideration for publication elsewhere.
All authors named in each manuscript would be required to sign a form (to be supplied by the Editor) so that they may retain their copyright in the article but to assign to us (the Publishers) and its licensees in perpetuity, in all forms, formats and media (whether known or created in the future) to (i) publish, reproduce, distribute, display and store the contribution, (ii) translate the contribution into other languages, create adaptations, reprints, include within collections and create summaries, extracts and/or abstracts of the contribution, (iii) create any other derivative works(s) based on the contribution, (iv) to exploit all subsidiary rights in the contribution, (v) the inclusion of electronic links from the contribution to third party material where-ever it may be located, and (vi) license any thrid party to do any or all of the above.
Main Article Content
Antibacterial synergy of Tritirachium oryzae-produced silver nanoparticles with different antibiotics and essential oils derived from Cupressus sempervirens and Asteriscus graveolens (Forssk)
Abstract
Purpose: To carry out eco-friendly biosynthesis of fungi-derived silver nanoparticles (AgNPs) and investigate their antibacterial synergies with essential oils (EOs) of Asteriscus graveolens (Forssk.) Less. and Cupressus sempervirens.
Methods: Biosynthesis of AgNPs was carried out using a cell-free filtrate of Tritirachium oryzae. The biosynthesized AgNPs characteristics were assessed using different methods, including ultravioletvisible spectrophotometry (UV), Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) with energy-dispersive x-ray spectroscopy (EDS) and transmission electron microscopy (TEM).
Results: Obvious synergistic effects were observed between AgNPs and chloramphenicol, vancomycin, nitrofurantoin or tetracycline with Pseudomonas aeruginosa, through increases in fold area of inhibition (IFAs) within the range of 2.4 to 9.0. Synergistic interactions were also seen between AgNPs and the antibiotics used, depending on the strain. Increase in IFA ranged from 1- to 3-fold for S. aureus, E. coli and P. aeruginosa. Similarly, combinations of AgNPs, EO of A. graveolens and cefotaxime, nitrofurantoin or amoxicillin against P. aeruginosa led to 10-, 3- and 10-fold synergy, respectively. In contrast, the use of AgNPs and trimethoprim, tetracycline or amoxicillin against E. coli led to 1 to 6-fold synergy. The best synergistic capacity resulted from AgNPs and the EO of C. sempervirens and trimethoprim against S. epidermidis, which yielded 29-fold increase in IFA. The use of combination of AgNPs and vancomycin against P. aeruginosa led to 16.4-fold enhancement of IFA.
Conclusion: The findings can potentially lead to the development of a new perception of antibacterial agents (innovative medications) involving the incorporation of nanoparticles (NPs) or new materials that potentially synergize with antibiotics, NPs and the EOs of different plants.
