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Published:
Jul 20, 2021Keywords:
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Main Article Content
Curcumin inhibits viability and promotes apoptosis by modulating miR-17/caspase-9 pathway in colorectal cancer
Abstract
Purpose: To investigate the mechanism of curcumin effect on colorectal cancer cells.
Methods: The miR-17-5p and caspase-9 were measured using quantitative real time polymerase chain reaction (qRT-PCR) and western blotting in colorectal cancer tissues or cells with or without curcumin treatment. The binding sites between miR-17-5p and caspase-9 were predicted by TargetScan and verified by luciferase assay. The miR-17-5p mimics were transfected into colorectal cells to determine its effects. The overexpressing miR-17-5p mimics and a caspase-9 plasmid were co-transfected into colorectal cells to explore the underlying mechanism. In addition, an in vivo experiment was performed in a mouse model after injection of HCT116 cells to determine the role of curcumin.
Results: MiR-17-5p was upregulated in colorectal cancer tissues and cells. Curcumin treatment inhibits viability and induces apoptosis of colorectal cancer cells. MiR-17-5p inhibits viability and induces apoptosis of colorectal cancer cells by regulating the expression of caspase-9. Mechanism studies showed that curcumin induced colorectal cell apoptosis by regulation of caspase-9 expression via miR17-5p. Finally, the animal results demonstrated the anti-tumor activity of curcumin in vivo.
Conclusion: The findings of this study indicate that curcumin suppresses cell apoptosis and induce cell viability by regulating miR-17-5p and caspase-9 in colorectal cancer.
