Article Sidebar
Published:
Jun 22, 2021Keywords:
Article Details
Submission of a manuscript to this journal is a representation that the manuscript has not been published previously and is not under consideration for publication elsewhere.
All authors named in each manuscript would be required to sign a form (to be supplied by the Editor) so that they may retain their copyright in the article but to assign to us (the Publishers) and its licensees in perpetuity, in all forms, formats and media (whether known or created in the future) to (i) publish, reproduce, distribute, display and store the contribution, (ii) translate the contribution into other languages, create adaptations, reprints, include within collections and create summaries, extracts and/or abstracts of the contribution, (iii) create any other derivative works(s) based on the contribution, (iv) to exploit all subsidiary rights in the contribution, (v) the inclusion of electronic links from the contribution to third party material where-ever it may be located, and (vi) license any thrid party to do any or all of the above.
Main Article Content
Neferine induces apoptosis of pancreatic cancer cells through p38 MAPK/JNK activation
Abstract
Purpose: To investigate the functional role of neferine on pancreatic cancer (PC) cell apoptosis.
Methods: The pancreatic cell line, PANC-1 cells, was exposed with different concentration of neferine. CCK8 and flow cytometry (Cell counting kit-8) were carried out to detect cell proliferation and apoptosis. Protein expression was evaluated by western blot.
Results: Neferine suppressed cell viability and caused cell cycle arrest of pancreatic cells in a dosedependent way. The effect of neferine on pancreatic cells was dependent on its ability to regulate the expression of cyclin E, cyclin D1, p21, cleaved caspase-3, cleaved PARP, Bcl-2 and Bax. In addition, neferine treatment induced the apoptosis of PANC-1 cells via promoting the activation of p38 MAPK/JNK signaling pathway.
Conclusions: Neferine inhibits cell viability and proliferation, and promotes apoptosis of PC cells by activating p38 MAPK/JNK signaling pathway. These results indicated the potential therapeutic effect of neferine in the treatment of PC.
